PPAR gamma Pro 12Ala and C161T polymorphisms, but not PPAR alpha L162V, are associated with osteoporosis risk in Turkish postmenopausal women

Sirin O. K., Aydogan H. Y., Uyar M., Can A.

ISTANBUL JOURNAL OF PHARMACY, cilt.49, sa.1, ss.14-19, 2019 (ESCI) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 49 Sayı: 1
  • Basım Tarihi: 2019
  • Doi Numarası: 10.26650/istanbuljpharm.2019.18005
  • Dergi Adı: ISTANBUL JOURNAL OF PHARMACY
  • Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI), TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.14-19
  • Anahtar Kelimeler: Peroxisome protiferator-activated receptor, bone mineral density, osteoporosis, polymorphism, ACTIVATED RECEPTOR-GAMMA, BONE-MINERAL DENSITY, PRO12ALA POLYMORPHISM, GENE POLYMORPHISMS, EXPRESSION, FRACTURE, EXON-6, BETA
  • İstanbul Üniversitesi Adresli: Evet

Özet

Stimulation of peroxisome proliferator-activated receptors (PPARs) causes mesenchymal stem cells of the human bone marrow differentiate into adipocytes instead of osteoblasts leading to a decreased number of osteoblasts and a decrease in bone mineral density IBMD). Thus, PPARs may have impacts on bone metabolism. 224 postmenopausal women (171 osteoporotic and osteopenic, 53 healthy control) were included in this study. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and agarose gel electrophoresis techniques were performed to detect PPAR alpha L162V and PPAR gamma Pro12Ala/C161T polymorphisms. The distribution of PPAR gamma Pro12Ala genotype and allele frequencies was not statistically different in control and patient (osteopenic+osteoporotic) groups (p>0.05). However, in the patient group, subjects with "Pro12Pro" genotype had lower lumbar spine (L1-L4) BMD values than those with "Ala" allele (p<0.05). The frequency of PPAR gamma C161T "CC" genotype was higher in the patient group when compared with that in the control group lp<0.051. There were no significant associations between the genotype and allele frequencies of PPAR gamma C161T PPAR alpha L162V and BMD values (p>0.05). We suggested that PPAR gamma Pro12Ala and C161T gene variants might be contributing factors in the development of osteoporosis.