The effect of a seed coating with Origanum vulgare essential oil on Clavibacter michiganensis subsp. michiganensis

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Yılmaz M., Baysal Ö., Silme R. S.

PLANT PROTECTION SCIENCE, cilt.57, sa.3, ss.217-225, 2021 (SCI-Expanded)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 57 Sayı: 3
  • Basım Tarihi: 2021
  • Doi Numarası: 10.17221/177/2020-pps
  • Dergi Adı: PLANT PROTECTION SCIENCE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Agricultural & Environmental Science Database, BIOSIS, CAB Abstracts, Veterinary Science Database, Directory of Open Access Journals
  • Sayfa Sayıları: ss.217-225
  • İstanbul Üniversitesi Adresli: Evet

Özet

The study aimed to evaluate the efficacy of a seed film coating with Origanum vulgare Linnaeus essential
oil (EO) against Clavibacter michiganensis subsp. michiganensis (Cmm) on tomatoes. Tomato seeds (cv. Rio Grande)
coated with different doses of EO derived from O. vulgare were inoculated with Cmm (1.8 × 108 CFU/mL). O. vulgare's
EO showed a remarkable inhibition effect on the Cmm growth. The EO effect against Cmm was determined based
on the parameters, such as the inhibition zone and bacterial population in a seed. The GC-MS analysis of EO showed
that carvacrol is the major component (at 74.05%), which may inhibit the bacterial growth. Later, we have expanded
our studies to determine the inhibitory effect of the EO's mode of action on the pathogenic bacteria with a molecular
docking analysis based on the molecular protein-ligand interaction. The results showed that carvacrol has a strong
interaction with the bacterial expansin protein (PDB 4JJO) of Cmm and the qPCR analyses confirmed the effect of the
O. vulgare treatment against Cmm. This original approach has the prominent potential to prevent seed transmission
of Cmm for seed quality in the world, suggesting a method for paving the way for Cmm disease management.