Detection of beta-lactam antibiotic resistance in aquatic Enterobacteriaceae isolates

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Zorbozan H., Kimiran A.

WATER SUPPLY, 2022 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Basım Tarihi: 2022
  • Doi Numarası: 10.2166/ws.2022.385
  • Dergi Adı: WATER SUPPLY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, PASCAL, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Compendex, EMBASE, Environment Index, Geobase, ICONDA Bibliographic, Pollution Abstracts, Veterinary Science Database, Directory of Open Access Journals
  • Anahtar Kelimeler: antibiotic susceptibility, aquatic environments, beta-lactamase, Enterobacteriaceae, resistance genes, ESCHERICHIA-COLI, EXTENDED-SPECTRUM, MOLECULAR CHARACTERIZATION, KLEBSIELLA-PNEUMONIAE, METAL RESISTANCE, WASTE-WATER, GENES, DIVERSITY, SUSCEPTIBILITY, EPIDEMIOLOGY
  • İstanbul Üniversitesi Adresli: Evet

Özet

Production of the beta-lactamase enzymes is the primary resistance mechanism caused by uncounscious consumption of the beta-lactam group of antibiotics in Enterobacteriaceae. Combined disc tests were performed for extended-spectrum beta-lactamase (ESBL) confirmation (n =15) and class C beta-lactamase (AmpC) confirmation (n = 6) after the disc diffusion test was applied in total of 27 Enterobacteriaceae strains from Western Black Sea, Lake Manyas and Lake Iznik. Plasmid-mediated bla(TEM), bla(SHV), bla(CTX-M), bla(IMP) genes, CIT, MOX, FOX gene groups and chromosomal bla AmpC gene of E. cloacae were investigated with polimerase chain reaction (PCR). Three E. coli strains were shown to produce ESBL while one E.coli and three E. cloacae strains were confirmed for AmpC production by disc tests. bla(TEM) (n= 2), bla(SHV) (n = 4), bla(CTX-M) (n = 2), bla(TEM) + bla(SHV) (n = 1), bla(CTX-M) + CIT (n = 1), bla(SHV )+ CIT (n = 1) genes and/or gene groups were detected in eleven E. coli isolates; while bla(SHV) (n = 3) was observed in three Klebsiella strains (two K.oxytoca and one K.pneumoniae). With this study, aquatic environments of Turkey were proved to be the reservoirs of antibiotic resistance determinants of plasmid-mediated beta-lactamases in environmental wild-type bacteria transmitted by horizontal gene transfer.