Development and validation of a sensitive spectrofluorimetric method for the determination of amoxapine in human plasma and urine

Karasakal A., Ulu S. T.

LUMINESCENCE, cilt.29, sa.3, ss.284-287, 2014 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 29 Sayı: 3
  • Basım Tarihi: 2014
  • Doi Numarası: 10.1002/bio.2541
  • Dergi Adı: LUMINESCENCE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.284-287
  • İstanbul Üniversitesi Adresli: Evet

Özet

A selective and sensitive spectrofluorimetric method was developed and validated for the determination of amoxapine in human plasma and urine. The developed method is based on labeling with 5-dimethylaminonaphthalene-1-sulfonyl chloride (dansyl chloride) and monitoring at 397nm (excitation)/514nm (emission). The method was validated for linearity, limit of detection (LOD), limit of quantification (LOQ), precision, accuracy, recovery and robustness. The calibration curves were linear over a concentration range of 250-2500 and 50-1250ng/mL for plasma and urine, respectively. The LOD values were calculated to be 13.31 and 13.17ng/mL for plasma and urine, respectively. The proposed method was applied to study of amoxapine in human plasma and urine. Copyright (c) 2013 John Wiley & Sons, Ltd.