Akademik Veri Yönetim Sistemi
38th NATIONAL CARDIOLOGY CONGRESS,, Antalya, Türkiye, 10 - 13 Kasım 2022, cilt.26, ss.157
Background and Aim: Coronary artery disease (CAD) is one of the most important public health problems. It is of great importance to determine the underlying mechanisms of CAD and non-invasive biomarkers that will provide information about the presence and severity of CAD. Thus, the determination of microRNAs (miRNA) released from cells into the circulation in pathological conditions has emerged as an aim. In this study, it was aimed to determine the circulating levels of miR-126, miR-210, let-7g and miR-326 in CAD and control groups.
Methods: The individuals who underwent coronary angiography and included in the study were classified according to their clinical status as non-CAD (≤30 stenosis, n=55), stable angina pectoris (SAP) (n=48), unstable angina pectoris (UAP) (n=46) and myocardial infarction (MI) (n=36) patients. Circulating miR-126, miR-210, let-7g and miR-326 expression levels were determined in serum samples using the quantitativeReal-Time PCR method. Possible target genes of miRNAs and the pathways in which these genes are enriched were investigated by performing bioinformatic analyzes.
Results: Circulating levels of let-7g are found to increase in the MI group compared to UAP, while miR-210 levels are lower in MI patients compared to the other groups. Moreover, miR-126 levels are decreased in UAP and MI patients relative to the non-CAD group. The circulating levels of let-7g, miR-126, and miR-210 are correlated with lipid levels and ratios. In addition, ROC curve analyses of miR-126 and miR-210 are shown promising results as biomarkers. Moreover, in silico analyses revealed that putative targets of the selected miRNAs are associated with CAD development and relevant pathways.
Conclusions: In this study, circulating of let-7g, miR-210, and miR-126 were found differentially expressed in MI group than others and miRNAs were found in correlation with lipid levels and ratios. In conclusion, the results of the study suggest that circulating let-7g, miR-126-3p, and miR-210-3p have potential as biomarkers and their mechanism of action should be elucidated with further functional studies.