Akademik Veri Yönetim Sistemi
2nd International Molecular Biosciences PhD and Postdoc Conference, Cologne, Almanya, 23 - 25 Kasım 2023, ss.71
It is thought that the activation of the kynurenine pathway causes inflammation and
plays an important role in obsessive-compulsive disorder (OCD) pathogenesis.
Cytochrome P450 (CYP) enzyme activity has been shown to be modified by
inflammatory cytokines. The members of μ of GSTs of the GSTs multigenic family show
a common polymorphism that is characterized by the complete deletion of the gene. It
is known that homozygous deletions (null genotype) result in the absence of catalytic
activity of the GST enzyme. The null genotypes of GSTs have been associated with
oxidative stress-related diseases such as cardiovascular, epilepsy, and psychiatric
diseases. We aimed to examine the relationship between pro-inflammatory cytokine
interleukin-1beta (IL-1β), anti-inflammatory interleukin-10 (IL-10) and interleukin-4 (IL-4),
serum cytochrome P450 (CYP1A1) and glutathione S-transferase1 (GSTM1) null
genotype in OCD. A hundred people with OCD and fifty healthy controls were included in
our study. Enzyme-linked Immunosorbent Assay (ELISA) was used to determine serum
IL-1β, IL-4 and CYP1A1 levels. GSTM1 present/null genotyping was performed using
polymerase chain reaction (PCR) from isolated DNA samples. We found that IL- 1β levels
were significantly increased in the OCD group, whereas IL-10 was decreased (p < 0.001,
for both). Serum IL-4 levels and GSTM1 present/null genotype distributions were
comparable between groups (p > 0.05, for both). IL-10 levels were significantly different
in the OCD group carrying the GSTM1 null allele compared to the controls (p < 0.01).
Inflammation and having the GSTM null allele may contribute to the pathology of OCD.