Akademik Veri Yönetim Sistemi
Diğer, ss.1-45, 2023
Botrytis cinerea (Bc), a plant pathogenic fungus, causes gray mold disease and rapidly
develops resistance to fungicides in cultivation areas. In this study, a
gram-negative, soil-borne bacterial colony was isolated and exposed to phenol
vaporization for 2 days. The colonies treated with phenol displayed restricted
growth of Bc's spores. The highest antibiosis effect was further confirmed using
agar bioassays based on their ability to stably suppress pathogen growth. In vitro assays with the colonies showed an 84% inhibition of pathogen growth
at 7 dpi using a one-layer agar diffusion test, and a 70% inhibition using a
double-layer agar diffusion test, compared to the control plates. In vivo tests involving fruit inoculation, bacterial suspension, and filtrate
showed a significant suppression of the pathogen's mycelium growth at 11 and 14
dpi, compared to the control group. The bacterial strain was identified as Serratia fonticola (EBS19) through whole genome sequence analysis. Comparative genomic
analysis using the KEGG pathway database revealed genes encoding enzymes that
play a role in inhibiting pathogen growth by S. fonticola. Additionally, BIOLOG
analyses identified specific carbon sources utilized by the bacterial strain.
This information could be advantageous for formulating an effective
biopreparate composition, ensuring the stability of the bacterial strain's
population.
Computational studies were conducted to model the interaction between the stress regulator protein (BAG1) of the pathogen and the bacterial glycoside hydrolase enzyme. The predictive modeling results could complement the unclear property of bacterial glycoside hydrolase enzyme activity and its inhibitory effect on the pathogen's stress regulator protein.