EPICARDIAL AND VISCERAL ADIPOSE TISSUE EXPRESSION LEVELS OF MIR-1247-5P AND PGC-1Α GENE AREASSOCIATED WITH OBESITY

Çoban N., Doğan N., Özuynuk-Ertuğrul A. S., Geyik F., Kırşan C. B., Yıldız C. E., ...Daha Fazla

91st European Atherosclerosis Society Congress, Mannheim, Almanya, 21 - 24 Mayıs 2023

  • Yayın Türü: Bildiri / Yayınlanmadı
  • Basıldığı Şehir: Mannheim
  • Basıldığı Ülke: Almanya
  • İstanbul Üniversitesi Adresli: Evet

Özet

Background and Aims

Epicardial adipose tissue (EAT) is a visceral adipose tissue (VAT) that surrounds the heart and coronary arteries. It was previously found that during coronary artery disease (CAD) progression, EAT miRNA and mRNA expression profile is changed. In this study, it is aimed to identify the associations between known CAD risk factors and expression levels of miRNA and mRNAs.

Methods

Epicardial adipose tissue (EAT) samples (63 CAD; 30 non-CAD), and visceral adipose tissue (VAT) samples from 65 individuals (46 CAD; 19 non-CAD) were collected. Anthropometric and biochemical measurements are done. PPARGC1A which encodes PGC-1α and miR-1247-5p expression levels were examined in EAT and VAT samples using real-time PCR.

Results

Body mass index (BMI) and EAT miR-1247-5p expression levels were negatively correlated in the study population (p=0.011), male (p=0.004), and in the CAD group (p<0.001). In addition, PPARGC1A expression levels in EAT samples were also negatively correlated with BMI in the study population (p=0.024) and in the CAD group (p=0.027). In male individuals, EAT PPARGC1A expression levels were negatively correlated with BMI (p=0.003) and fasting glucose levels (p=0.026). In addition, VAT PPARGC1A expression levels were negatively correlated with BMI in the study population (p=0.002), male (p=0.010), and in the CAD group (p=0.001).

Conclusions

The results obtained in the study show that the EAT and VAT expression levels of miR-1247-5p and PPARGC1A which is the key regulator of cellular energy metabolism are negatively associated with obesity. Moreover, this is the first study that demonstrated the association between EAT miR-1247-5p expression levels and BMI.