DETERMINATION OF BARLEY HYBRIDS WITH MOLECULAR MARKERS

ART S., BILGEN G., COBANOGLU G., GUREL F., GOZUKIRMIZI N.

CEREAL RESEARCH COMMUNICATIONS, cilt.23, sa.3, ss.229-234, 1995 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 23 Sayı: 3
  • Basım Tarihi: 1995
  • Dergi Adı: CEREAL RESEARCH COMMUNICATIONS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.229-234
  • İstanbul Üniversitesi Adresli: Evet

Özet

Wild lines and cultivars of barley (Hordeum vulgare, L. cvs. Kaya, Quantum, Tokak, Yercil and Cumhuriyet) and their hybrids were characterized by random amplified polymorphic DNA assay. DNA's isolated from parents and F-1 hybrids were amplified using ten base long primers by a polymerase chain reaction (PCR). Hybrids giving selective banding patterns from both the cultivars and wild parents were taken as real hybrids. This technique is convenient for plant breeders since it is rapid, sensitive and inexpensive.